The Research Behind DUTCH

Evidence Based. Provider Tested. Patient Approved.

DUTCH is the most advanced hormone test for functional and integrative medical practitioners, especially when monitoring (b)HRT. Precision Analytical is a CLIA certified laboratory run by a small team of dedicated scientists with combined experience of over 50 years in performing laboratory tests and developing novel testing methods. We use the most accurate method available (LC-MS/MS and GC-MS/MS) and our tests are used by thousands of healthcare providers and their patients all over the world.

DUTCH Research Inquiries

We welcome collaborations with researchers, healthcare companies, and clinicians, including clinical trials which require a CLIA-certified testing laboratory. If you are interested in collaborating with DUTCH on a research project, please visit this page and fill out the online form. A member of our staff will contact you shortly by phone or email.

Peer-Reviewed Research

Scientific literature has much to say about the viability of measuring hormones in urine. DUTCH's first peer-reviewed journal submission was published in 2019 in BMC Chemistry. It was completed collaboratively with the University of Southern California Reproductive Endocrine Clinical Laboratory and shows that analysis of urine samples dried on filter paper provides results correlating with serum (blood) results. The research also shows a collection of four samples during the day (4-spot method) can be substituted for a 24-hour collection and the analysis of urine from dried samples is equivalent and is as adequately stable as liquid urine samples.

In a newly released publication, DUTCH was featured in a study to determine the utility of dried urine sampling in obtaining measures of cortisol and cortisol metabolites. Additional aims were to evaluate if a 4-spot urine collection is representative of a 24-hour urine collection and if expected diurnal cortisol patterns can be observed in samples from both urine and saliva.

More peer-reviewed journal submissions featuring DUTCH are in process.

Research Collaborations and Clinical Trials

DUTCH testing was also recently used in tandem with the Oura Ring to explore a potentially new method for predicting and defining the fertile window for women. This study was completed collaboratively with researchers from The Helen Wills Neuroscience Institute, University of California Berkley. Mark Newman, MS, president and founder of Precision Analytical Inc. was a coauthor of this paper which demonstrates how ultradian rhythms of distal body temperature and heart rate variation, measured using the Oura Ring, were able to anticipate the LH surge “at least two days prior to its onset in 100% of individuals.” This finding has the potential to lead to the full development of a novel fertility awareness tool that would be extremely useful for both women trying to conceive, as well as those seeking a natural contraception method.

DUTCH-Based Research

It is important to remember that many of the concepts used in the DUTCH test® are already established in the literature. The relevance, for example, of measuring estrogen metabolites in spot urine samples is found both in the scientific literature and is offered by other labs. For this test, we are simply using dried samples, which show excellent correlation to liquid urine and great stability for months at room temperature. One challenge to serum hormone testing is the fact that reproductive hormones change somewhat unpredictably throughout the day. Filicori, Butler and Crowley showed in the Journal of Clinical Investigation that serum progesterone values are highly variable throughout the day in premenopausal women. One advantage of the DUTCH collection and testing methodology is that the four urine samples used represent over half of the day, instead of just one moment in time.

Precision Analytical Inc. maintains a transparent approach to discussing the strengths and challenges of all testing modalities. Some competing labs and even a few healthcare practitioners have circulated questions about the DUTCH Test®. We asked ourselves many of the same questions when we began exploring the DUTCH concept. These papers give an overview of the challenges and questions to using dried, spot-urine samples for hormone analysis, and address some of the questions regarding DUTCH, hormone testing in general, and even hormone replacement therapy:

DUTCH Clinical Validation Paper

Literature Review and Best Practices

BMC Chemistry, https://doi.org/10.1186/s13065-019-0539-1
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Oura Ring
Clinical Validity of DUTCH Thumbnail REF022720
Transdermal Estradiol Dosing Thumbnail
Research-Inquiry